ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (11): 1799-1808.doi: 10.11843/j.issn.0366-6964.2014.11.009

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Studies on Dietary Methionine Requirement of Jinghong Laying Hens from 5- to 8-week-old

SONG Dan1,2,LI Lian-bin1,2，ZHOU Liang1，YUE Hong-yuan1,GAO Yu-peng2,WU Shu-geng1*，QI Guang-hai1*   

  1. (1.National Engineering Research Center of Biological Feed，Feed Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081,China；2.College of Animal Science and Technology,Northwest A & F University,Yangling 712100,China)
  • Received:2014-03-25 Online:2014-11-23 Published:2014-11-23

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Abstract:

The modulation of dietary methionine(Met) levels on Jinghong laying hens from 5- to 8-week-old was studied in this study.Three hundred 29-day-old Jinghong laying hens were randomly allotted to 5 dietary treatments，and each treatment contained 5 replicates with 5 pens per replicate and 12 birds per pen.The methionine levels were 0.30% (0.30% Met)，0.37% (0.37% Met)，0.44% (0.44% Met)，0.51% (0.51% Met) and 0.58% (0.58% Met)，respectively.The experiment lasted for 28 days.The result showed that：1)Dietary Met levels had no significant influence on average daily gain and average daily feed intake (P>0.05); feed/gain ratios and body weight were significantly affected by dietary Met levels(P<0.05)，and the birds in 0.44% Met group were the best (608.67 g and 3.13∶1);community evenness also showed the tendency of a quadratic curve of rising，and the birds in 0.37% Met group were the best(91.67％)；the pectorales index，leg muscles index，chest width and shinbone length were significantly affected by dietary Met levels (P<0.05)，with a quadratic curve of rising tendency，and the birds in the 0.44% Met group had the best pectorales index and shinbone length.2) By the end of 6th week old，the dietary Met levels had significant influences on the spleen index，thymus index and bursa of fabricius index (P<0.05);By the end of 8th week old，no significant effects were found on thymus index or bursa of fabricius index (P>0.05),but the spleen index was significantly affected (P<0.05)，and the birds in 0.44% Met group had the best thymus index with 0.26%，the birds in 0.37% Met group had the best bursa of fabricius index.3) Dietary Met levels significantly affected the pancreas，duodenum index，jejunum index and jejunum length (P<0.05).With the increasing of methionine，all the indexes showed the rise-fall tendency.4) Dietary Met levels had significant effect on serum uric acid and alkaline phosphatase levels (P<0.05).The level of uric acid and alkaline phosphatase in the 0.44% Met group was significantly higher than that of the other groups (P<0.05).Dietary Met levels had no significant effects on serum urea nitrogen，albumin，total protein，follicle stimulating hormone，luteinizing hormone and growth hormone (P>0.05).5) According to quadratic curve estimation of the weight，feed/gain，community evenness，chest muscle，leg muscle，chest width and shinbone length,the optimal methionine levels were 0.43%，0.44%，0.38%，0.45%，0.43%，0.41% and 0.40%，which could be averaged to 0.42%.To sum up，the optimal Met requirement for Jinghong laying hens from 5- to 8-week-old is 0.42%.

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